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      Sensitivity to drugs of chemotherapy of cancer stem cells of human pancreatic carcinoma

      2012-08-15 00:54:32WangYuhe
      外科研究與新技術(shù) 2012年1期

      /Wang Yuhe

      (王育和,Dept Surg,Beijing Xiaotangshan Hosp,Beijing 102211)…∥Chin J Hepatobil Surg.-2011,17(11).-919~923

      ObjectiveTo explore the sensitivity of cancer stem cells to chemotherapy in human pancreatic carcinoma.MethodsPANC-1 ceils were cultured in an incubator filled with 5%CO2 at a temperature of 37℃,and were labeled with Hoechst 33342.The SP analysis and sorting were performed using a FACSVantage SE.RTPCR was performed to detect the expression of human CD133 ABCG2 and Notch1.SP and non-SP cells from the PANC-1 cell line were treated with 5-fluorouracil(5-FU;1,10,or 100 μg/ml)or gemcitabine(10,100,or 1000μg/ml),and the cell viability was determined using the MTT assay.The sensitivity of sorted tumor cells to chemotherapeutics was determined in NOD/SCID mice model.ResultsSP cells were found to have higher drug-resistance both in vivo and in vitro and higher levels of mRNA expression for CD133,ABCG2 and Notch1,when compared to non-SP cells.The xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133± cells than the untreated group.ConclusionThe SP of PANC-1 pancreatic carcinoma cells are enriched with highly gemcitabine-resistant CSCs and determine the carcinogenesis of the PANC-1 pancreatic carcinoma cells.16 refs,4 figs.

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