• 
    

    
    

      99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看 ?

      Study of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptotic and inflammatory gene expressions in colon cancer cell line

      2012-08-15 00:54:32YuHongbin
      外科研究與新技術(shù) 2012年1期

      /Yu Hongbin

      (俞宏斌,Dept Anus Intest,Peop Hosp Huzhou,Zhejiang Huzhou 313000)…∥Chin J Gastrointest Surg.-2011,14(8).-803 ~806

      ObjectiveTo study the effect of recombinant tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)on the expression of apoptosis and inflammatory related genes in human colon cancer cell line HCT-116.MethodsAfter 24-hour treatment with recombinant human TRAIL protein,the expressions of apoptosisrelated genes(Bcl-2,Bad,caspase-3,and caspase-8)and inflammation-related genes(TNF-α,IL-1β,and COX-2)were measured by real-time PCR and appropriate kits in HCT-116.ResultsAfter treatments of 10 μg/L and 100 μg/L recombinant human TRAIL proteins,the apoptotic rates of HCT-116 cells were 27.4%and 45.9%,respectively.Expressions of anti-apoptosis gene Bcl-2,pro-apoptosis gene Bad and apoptotic markers caspase-3 and caspase-8were significantly up-regulated,which was more significant in the group of 100μg/L treatment(P<0.05).Moreover,after TRAIL treatments,expressions of inflammation-related genes TNF-α,IL-1,COX-2were also dramatically increased,and 100 μg/L treatment group showed higher up-regulation(P < 0.05).ConclusionRecombinant TRAIL protein induces both apoptosis and inflammation of human colon cancer cells.7 refs,4 figs,1 tab.

      郧西县| 水城县| 文成县| 南昌市| 河间市| 揭阳市| 札达县| 西乌珠穆沁旗| 澄城县| 平安县| 丰宁| 长寿区| 大悟县| 泽州县| 西峡县| 宝兴县| 安阳市| 定结县| 东海县| 东乌珠穆沁旗| 柘城县| 资兴市| 定南县| 通河县| 宁远县| 大英县| 宁晋县| 上杭县| 阜阳市| 巩留县| 惠安县| 陕西省| 静乐县| 廊坊市| 福海县| 石狮市| 南陵县| 阜新市| 龙游县| 辉南县| 稻城县|