• 
    

    
    

      99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看 ?

      Interference of invasion and metastasis of colorectal cancer cells by EMS1-siRNA encoded by a recombinant lentiviral vector

      2012-08-15 00:54:32QiuFeng
      外科研究與新技術(shù) 2012年1期

      /Qiu Feng

      (邱烽,Dept Gastrointest Surg,Affil Hosp Nantong Univ ,Jiangsu Nantong 226001)…∥Chin J Gastrointest Surg.-2011,14(8).-887~891

      ObjectiveTo explore the effect of Cortactin on invasion and metastasis of human colorectal cancer cells through silencing the expression of Cortactin by transfecting colorectal cancer cells with EMS1-siRNA encoded by a recombinant lentiviral vector.MethodsEMS1-siRNA lentiviral vector and negative control lentiviral vector were both transfected into HCT8 cells after virus packing,then the expression of Cortaction of the cells was examined by Western blot to determine the inhibited level of Cortactin.The cells were defined as three groups:EMS1-siRNA transfecting cells,negative control transfecting cells and untransfecting cells.Cell invasion was evaluated with Transwell body.In addition,cells metastasis was observed in animal models.Thirty nude mice were evenly divided into three groups and all the mice were injected through tail vein with 1 ×107 cells/0.2 ml per animal for each group with the three kinds of cells.The mice were sacrificed 6 weeks after injection and examined for lung metastases development.ResultsThe expression of Cortactin and the ability of invasion of the EMS1-siRNA transfecting cells were all decreased.Lung metastasis rates of EMS1-siRNA transfecting group,neg-ative control group and untransfecting group were 20%,80%and 80%,respectively.ConclusionsiRNA silencing of Cortactin expression can decrease the colorectal cancer cells invasion,and can also decrease the cells’implantation metastasis in animals.10 refs,5 figs.

      揭西县| 阜城县| 珠海市| 高雄县| 双鸭山市| 黄大仙区| 屏东县| 尼木县| 恩平市| 德保县| 潜山县| 呼玛县| 永胜县| 青铜峡市| 共和县| 岗巴县| 崇左市| 益阳市| 古丈县| 太谷县| 福建省| 灵寿县| 临潭县| 苍溪县| 正阳县| 林甸县| 神农架林区| 漳州市| 墨玉县| 天长市| 舟曲县| 太保市| 竹溪县| 鲜城| 湘潭市| 瓮安县| 陈巴尔虎旗| 洪雅县| 科技| 凤城市| 磐石市|