• 
    

    
    

      99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看

      ?

      LW-AFC,a new formula derived from Liuwei Dihuang decoction,ameliorates behavioral and pathological deterioration via modulating the neuroendocrine-immune abnormalities in PrP-hAβ PPswe/PS1ΔE9transgenic mice

      2017-01-16 03:42:51JianhuiWANGXiLEIXiaoruiCHENGXiaoruiZHANGGangLIUJunpingCHENGYiranXUJuZENGWenxiaZHOUYongxiangZHANG

      Jian-hui WANG*,Xi LEI*,Xiao-rui CHENG*,Xiao-rui ZHANG,Gang LIU,Jun-ping CHENG,Yi-ran XU,Ju ZENG,Wen-xia ZHOU,Yong-xiang ZHANG

      (Department of Neuroimmunopharmacology,Beijing Institute of Pharmacology and Toxicology,State Key Laboratory of Toxicology and Medical Countermeasures,Beijing 100850,China)

      T2-23

      LW-AFC,a new formula derived from Liuwei Dihuang decoction,ameliorates behavioral and pathological deterioration via modulating the neuroendocrine-immune abnormalities in PrP-hAβ PPswe/PS1ΔE9transgenic mice

      Jian-hui WANG*,Xi LEI*,Xiao-rui CHENG*,Xiao-rui ZHANG,Gang LIU,Jun-ping CHENG,Yi-ran XU,Ju ZENG,Wen-xia ZHOU,Yong-xiang ZHANG

      (Department of Neuroimmunopharmacology,Beijing Institute of Pharmacology and Toxicology,State Key Laboratory of Toxicology and Medical Countermeasures,Beijing 100850,China)

      OBJECTIVETo investigate the effect of LW-AFC,a new formula of the main active components extracted from Liuwei Dihuang decoction,on treatment of Alzheimer disease(AD)in mouse models.METHODSAfter treatment LW-AFC,mice were cognitively evaluated in behavioral experiments.Neuron loss,amyloid-β(Αβ)deposition,and Αβ level were analyzed using Nissl staining,immunofluorescence,and an AlphaLISA assay,respectively.Multiplex bead analysis,a radioimmunoassay,immunochemiluminometry,and an ELISA were used to measure cytokine and hormone levels.Lymphocyte subsets were detected using flow cytometry.RESULTSLW-AFC ameliorated the cognitive impairment observed in APP/PS1 mice,including the impairment of object recognition memory,spatial learning and memory,and active and passive avoidance.In addition,LW-AFC alleviated the neuron loss in the hippocampus,suppressed Αβ deposition in the brain,and reduced the concentration of Aβ1-42in the hippocampus and plasma of APP/PS1 mice.LW-AFC treatment also significantly decreased the secretion of corticotropin-releasing hormone and gonadotropin-releasing hormone in the hypothalamus,and adrenocorticotropic hormone,luteinizing hormone,and follicle-stimulating hormone in the pituitary.Moreover,LW-AFC increased CD8+CD28+T cells,and reduced CD4+CD25+Foxp3+T cells in the spleen lymphocytes,down-regulated interleukin(IL)-1β,IL-2,IL-6,IL-23,granulocyte-macrophage colony stimulating factor,and tumor necrosis factor-α and-β,and up-regulated IL-4 and granulocyte colony stimulating factor in the plasma of APP/PS1 mice.CONCLUSIONLW-AFC ameliorated the behavioral and pathological deterioration of APP/PS1 transgenic micevia the restoration of the NIM network to a greater extent than either memantineor donepezil,which supports the use of LW-AFC as a potential agent for AD therapy.

      LW-AFC;Alzheimer disease;PrP-hAβPPswe/PS1ΔE9transgenic mouse;cognitive behavior;amyloid-β;neuron loss;neuroendocrine;lymphocyte subset;cytokine

      *Co-first author.

      The project supported by National Science and Technology Major Projects Initiative(2013ZX09508104);and National Natural Science Foundation of China(81473191)

      Wen-xia ZHOU,Tel:(010)66931625,Fax:(010)68211656,E-mail:zhouwx@bmi.ac.cn;Yong-xiang ZHANG,E-mail:zhangyx@bmi.ac.cn

      萝北县| 百色市| 珲春市| 永修县| 祁门县| 青龙| 铅山县| 张北县| 西充县| 永和县| 昭苏县| 金寨县| 江山市| 肇东市| 洪泽县| 新蔡县| 布尔津县| 河源市| 遵义县| 周至县| 天津市| 南和县| 五常市| 密云县| 缙云县| 弥渡县| 陆丰市| 遵义市| 林周县| 萍乡市| 华宁县| 河北区| 荆门市| 措美县| 锦屏县| 阳朔县| 浠水县| 松溪县| 东光县| 靖安县| 修文县|