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    Effects of total soy saponins on free radicals in the quadriceps femoris, serum testosterone,LDH,and BUN of exhausted rats

    2017-09-21 11:54:54ZhigngLiuRuixinNieYunLiuZhouhongLiChenxiYngZhengyingXiong
    Journal of Sport and Health Science 2017年3期
    關(guān)鍵詞:分?jǐn)?shù)線統(tǒng)一教師應(yīng)

    Zhigng Liu*,Ruixin NieYun Liu,Zhouhong Li,Chenxi Yng,Zhengying Xiong

    aSchool of Sport,Yuxi Normal University,Yuxi 653100,China

    bFaculty of Science,Yuxi Normal University,Yuxi 653100,China

    cSchool of Sport,Shaanxi Normal University,Xi’an 710062,China

    Effects of total soy saponins on free radicals in the quadriceps femoris, serum testosterone,LDH,and BUN of exhausted rats

    Zhigang Liua,*,Ruixin Niea,Yun Liub,Zhouhong Lib,Chenxi Yangb,Zhengying Xiongc

    aSchool of Sport,Yuxi Normal University,Yuxi 653100,China

    bFaculty of Science,Yuxi Normal University,Yuxi 653100,China

    cSchool of Sport,Shaanxi Normal University,Xi’an 710062,China

    Purpose:The aim of this study was to investigate the impact of total soy saponins(TS)on the free radical metabolism from the quadriceps femoris muscle,serum testosterone,lactate dehydrogenase(LDH),and blood urea nitrogen(BUN)in rats exercised to exhaustion.

    Methods:A one-time exhausted treadmill exercise session was used.Sprague-Dawley rats were divided into 4 groups:a control group—animals receiving noTS and no exercise(NTSNE),animals receivingTS but no exercise group(TSNE),animals receiving noTS but exercised to exhaustion group(NTSE),and animals receivingTS and exercised to exhaustion group(TSE).TheTSNE andTSE groups were fedTS at a dosage of 20 mg/kg body weight once per day for 2 weeks.The NTSE group was given a placebo,and the NTSNE group was not given any treatment.The NTSE and TSE groups were exercised at speed of 30 m/min on treadmill until exhausted.The exercise time and exercise distance were recorded when the rats became exhausted and the rats were then decapitated and anatomized immediately.A 10%homogenate of the quadriceps femoris tissue was prepared. The levels of superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),glutathione reductase(GR), reduced glutathione(GSH),total antioxidant capacity(T-AOC),LDH,BUN,and serum testosterone were tested.

    Results:TS signi ficantly increased the exercise time to exhaustion by 20.62%(p<0.05).The MDA levels were decreased signi ficantly in the TSNE group than in NTSNE group(p<0.05);the T-AOC levels increased signi ficantly in the TSNE group than in the other 3 groups(p<0.01, p<0.05,p<0.05).The LDH activity signi ficantly increased in the NTSE group than in TSNE group(p<0.05).The BUN levels signi ficantly increased in the NTSE group than in the other 3 groups(p<0.01,p<0.01,p<0.05),and signi ficantly increased in theTSE group than in NTSNE andTSNE groups(both p<0.01).The serum testosterone levels increased signi ficantly in theTSNE group than in the other 3 groups(all p<0.01). SOD,CAT,GSH-Px,GR,and GSH were not statistically different among the groups.

    Conclusion:TS can signi ficantly improve the exercised rats’serum testosterone level and antioxidant activity in their quadriceps femoris to varying degrees,decrease MDA and serum LDH and BUN levels,increase the exercise time,and delay the occurrence of the fatigue.

    ?2017 Production and hosting by Elsevier B.V.on behalf of Shanghai University of Sport.This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).

    Exercised rat;Free radical;Quadriceps femoris;Serum enzymes;Testosterone;Total soy saponins

    1.Introduction

    There are 2 free radical(FR)defense systems in the human body.One type is an enzymatic defense system such as superoxide dismutase(SOD),glutathione peroxidase(GSH-Px), catalase(CAT),and glutathione reductase(GR).The other is a non-enzymatic defense system such as vitamin C, vitamin E,and glutathione(GSH).Typically,the body keeps a dynamic balance between the generation and the removal of FR.However,under the condition of exhausted exercise,FR in the body increases signi ficantly.When the level of lipidperoxidation exceeds the body’s antioxidant capacity,this will result in the occurrence of oxidative stress and directly cause bio film injury,the degeneration of intracellular proteins,and lead to cell death,apoptosis,tissue damage,and disease.1

    Fig.1.Total soy saponins structure of A group.

    Fig.2.Total soy saponins structure of B,E,and DDMP groups.DDMP= 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one;TS=total soy saponins.

    TS have a variety of biological activities,such as antioxidant2and immune-enhancing activity.3,4They can also improve the rats’macrophage phagocytic capacity5and humoral and cellular immunity.3By inhibiting the activity of α-glucosidase6and α-amylase,7TS signi ficantly reduced the level of blood sugar in diabetic rats,and signi ficantly improved the glucose tolerance in both the diabetic and healthy rats.8TS also have signi ficant effects on anti-aging9and the inhibition of tumor cell DNA,10herpessimplexvirus(HSV-1),humancytomegalovirus (HCMV),polio virus,in fluenza virus,measles virus,mumps virus,and coxsackie virus.11–14Further anti-aging studies on human embryonic lung diploid fibroblasts in vitro con firmed that the cells treated with TS can grow extended to 80 generations, whereas the longest survival time of the control group was only until 51 generations.15TS also have a signi ficant capacity of anti-lipid peroxidation activity on plasma lipoprotein16and can prevent low-density lipoprotein cholesterol(LDL-C)from oxidizing and decrease their susceptibility to oxidation,thus hinderingtheconversionofLDL-Ctooxidizedlow-densitylipoprotein, which is an important factor in atherosclerosis risk.TS protected not only the heart,but also the vascular smooth muscle.TS can signi ficantly reduce the generation of lipid peroxides,protect endothelial integrity,and maintain the normal cardiovascular function.The aim of this study was to investigate the impact of TS on the free radical metabolism from the quadriceps femoris muscle,serum testosterone,lactate dehydrogenase(LDH),and blood urea nitrogen(BUN)in rats exercised to exhaustion.

    2.Methods

    2.1.Experimental design and subjects

    Thirty-two Sprague-Dawley(SD)healthy 2-month-old male rats were used(weight 190–210 g)and were provided care as directed by the Experimental Animal Center of the Medical School,Xi’an Jiao Tong University(animal certi ficate No.: Shaanxi Medical Animal No.08-005).This study was performed according to the international,national,and institutional rules considering animal experiments,clinical studies and biodiversity rights,and had been approved by Xijing Hospital Ethic Committee in Fourth Military Medical University.

    All rats were randomly divided into 4 groups:a control group—animals receiving no TS and no exercise(NTSNE), animals receiving TS but no exercise group(TSNE),animals receiving noTS but exercised to exhaustion group(NTSE),and animals receivingTS and exercised to exhaustion group(TSE). Eight rats from each group were fed in divided cages.The temperature varied from 22°C to 28°C,the relative humidity was 45%–65%,the cages were illuminated by natural light,the ambient noise was no higher than 45 dB and all rats had free access to water and basic rodent chow.

    North China Pharmaceutical Co.,Ltd(Shijiazhuang,China) provided TS with a purity of 90%and 10%ash.The rats were started on TS gavage after 3 days of adaptation to the environment.Each rat in the supplement groups(TSNE and TSE groups)was fed a 2 mL aliquot ofTS dissolved in normal saline at a fixed time between 9:00 a.m.and 9:30 a.m.,once per day for 2 weeks,with TS dosage of 20 mg/kg body weight.During the supplement gavage,the rats were weighed every 3 days and the dosage was adjusted in time according to the body weight. The NTSE group was fed the same volume of normal saline vehicle.The NTSNE(control)group received no treatments.

    2.2.Exhaustive exercise protocol

    An acute exhaustive exercise session was completed.The rats were not given any prior training;the NTSE and TSE groups underwent an acute exhaustive exercise session on thetreadmill only before dissection.The treadmill was horizontal and gradually increased to the predetermined exercise intensity (30 m/min)within 3 min.The treadmill speed was set at 10 m/min for the first minute,20 m/min for the second minute, and 30 m/min for the third minute.The exercise time to exhaustion and exercise distance for each rat were recorded.We judged whether the rats exercised to exhaustion according to the following criteria:the rats could not maintain a predetermined treadmill speed,squatted against the back wall of the treadmill lane on their buttocks,and both the current stimulus and the brush driving could not force the rats to continue exercising. The exhaustive behavior was characterized by shortness of breath,mental fatigue,and a prone nutation.

    2.3.Dissection and index test

    The rats were anesthetized with ether immediately after reaching a state of exhaustion and killed by decapitation.Blood was collected and the serum was separated after coagulation. The quadriceps femoris was removed immediately and the remaining blood was washed away with 4°C normal saline and placed in a clean culture dish,marked according to each group. The weight of the quadriceps femoris was measured and the quadriceps femoris was ground in 4°C normal saline.Quadriceps femoris tissue homogenates of 10%mass concentration were prepared and the supernatant was separated after centrifugation(7.99×g,5 min).Finally,antioxidant indicators,testosterone,LDH,and BUN were assayed accordingly.

    在實(shí)際教學(xué)中,以統(tǒng)一的分?jǐn)?shù)線作為所有人的目標(biāo)是不切實(shí)的,進(jìn)步是相對的。因此,教師應(yīng)引導(dǎo)不同層次的學(xué)生制定不同層次的目標(biāo),讓更多的學(xué)生獲得自我實(shí)現(xiàn)的成就感。

    2.4.Methods of testing

    The antioxidant indicators were tested with reagent kits provided by Nanjing Jiancheng Bioengineering Institute(Nanjing, China).SOD was tested by the xanthine oxidase method; malondialdehyde(MDA)was tested by the thiobarbituric(TBA) method;CAT was tested by the ultraviolet spectroscopy method; GSH-Px,GR,and GSH were tested by the dithiobis nitrobenzoic acid method;total antioxidant capacity(T-AOC)was tested by a spectrophotometry method;the testosterone was tested by the radioimmunoassay method;and the serum BUN and LDH were tested by an automatic biochemistry analyzer(Hitachi 7060;Hitachi Corporation of Japan,Tokyo,Japan;LabStar

    2.5;Beijing ZhifangTechnology Development Co.Ltd.,Beijing, China).

    A 721B spectrophotometer(Shanghai Jingke Instrument Co.,Ltd.,Shanghai,China),a 752B spectrophotometer(Shanghai Jingke),an FJ-2008Pγ radioimmunoassay counter(Xi’an Nuclear Instrument Factory,Xi’an,China),a Hitachi 7060 automatic biochemical analyzer,a TGL-16G refrigerated centrifuge(Flying Pigeon,Shanghai Anting Scienti fic Instrument Factory,Shanghai,China),a DK-98-1A water bath(Taisite, Tianjin City Taisite Instrument Co.,Ltd.,Tianjin,China)and a DSPT-202 treadmill(Duanshi,Shanghai Xinruan Information Technology Co.Ltd.,Hangzhou,China)were used.

    2.5.Data processing

    The experimental data were processed with statistical software SigmaStat Version 3.5(SYSTAT Software Inc.,San Jose, CA,USA)and the results were shown as mean±SD.A p<0.05 or p<0.01 was considered statistically signi ficant after a one-way analysis of variance(ANOVA)Student–Newman–Keuls test(S–N–K test).The exhaustive time was processed by a t test and the results of the t test were measured by Cohen’s d value.

    3.Results

    With velocity of 30 m/min,TS can signi ficantly improve time to exhaustion of the rats by 20.62%(86±12 min vs.103 ±19 min,p<0.05,Cohen’s d=1.07),which indicated that the present t test was trustworthy(Cohen standards,the t test has a small effect size,medium effect size,and large effect size when Cohen’s d value is 0.2,0.5,and>0.8,respectively. The Cohen’s d value of present t test was 1.07>0.8).

    Data presented in Table 1 show thatTS can improve the rats’antioxidant capacity of their quadriceps femoris to varying degrees.By S–N–K test of a one-way ANOVA,as compared with the NTSNE group,the MDA level signi ficantly decreased in the TSNE group(p<0.05).As compared with the NTSNE group(p<0.01),the NTSE group(p<0.05),and the TSE group(p<0.05),T-AOC activities signi ficantly increased in the TSNE group.

    Under the intervention ofTS,whether quiet or exercised,the activities of SOD,CAT,GSH-Px,GR,and GSH showed atendency to increase,but this difference was not statistically signi ficant.

    Table 1 Impact of TS on the antioxidant capacity of the quadriceps femoris in exercised rats(n=8 in each group,mean±SD).

    Table 2 Impact of TS on the serum LDH activity,BUN,and testosterone level in exercised rats(n=8 in each group,mean±SD).

    As shown in Table 2,by S–N–K test of a one-way ANOVA, LDH in NTSE group signi ficantly increased in response to exhausted exercise compared with the TSNE group(p<0.05). As compared with the NTSNE group(p<0.01),the TSNE group(p<0.01),and the TSE group(p<0.05),the BUN level signi ficantly increased in the NTSE group.As compared with both NTSNE and TSNE groups,the BUN level in TSE group signi ficantly increased(both p<0.01).The serum testosterone level signi ficantly increased in the TSNE group compared with the other 3 groups(p<0.01).

    4.Discussion

    The occurrence of fatigue is highly related to the working ability of the skeletal muscle.The FR was one of the key factors which resulted in the decline of skeletal muscle contractibility and the occurrence of fatigue.14According to the catastrophe theory of muscular fatigue,the fatigue can occur in any links from the cerebral cortex excitement to the contractile proteins of the skeletal muscle.15As the body exercises to exhaustion, approximately 2%O2of the body intake is converted to the superoxide anion FR in the way of one-electron reduction,16and the oxidative stress is enhanced.By the way of Haber–Weiss reaction,the superoxide anion FR and H2O2can generate hydroxyl radical,while the latter is more toxic to cells.The FR is mainly produced in the mitochondrion and endoplasmic reticulum of the skeletal muscle.The FR and its metabolite MDA can injure the quadriceps femoris cells,include lipid peroxidation,and attack nucleic acid and protein,and finally the biological functions of the cell are also damaged.17A mass of FR produced in exhaustive exercise not only consumed the antioxidant enzymes,but also attacked enzyme molecules,thus the synthesis and regeneration of antioxidant enzymes are affected.The results of the present study showed TS could signi ficantly improve the time to exhaustion in rats.This improved performance may be associated with the antioxidant effect of TS.As the experimental data showed in Table 1,TS can improve the rats’antioxidant capacity of their quadriceps femoris to varying degrees,and especially improve T-AOC activities and decrease MDA level and show a statistical difference.Compared with NTSNE group,the activity of T-AOC increased(p<0.05)while MDA level decreased(p<0.01),and the activity of SOD,GSH-Px,GR,and GSH showed a tendency of increase(but no statistical difference)inTSNE group.These results may be the outcome from a negative feedback regulation of the body:exhaustive exercise→oxidative stress↑→the compensation reaction of the body↑→antioxidative genetic expression↑→the activity of antioxidative enzyme↑→FR scavenging↑→FR level↓→MDA↓.We purpose that exercise provided a positive effect on the antioxidant capacity of the body,and this bene fit may be one of the factors by which exercise can provide an anti-aging effect.18

    Under the intervention of TS,the activity of SOD,CAT, GHS-Px,GR,GSH,and T-AOC in the TSE group rose slightly more than that found in the NTSE group while the MDA level was lower than in the NTSE group.The experimental results indicated that TS can improve the antioxidative enzymes activity of the quadriceps femoris,alleviate the oxidative stress,and delay the occurrence of fatigue.As the present data showed, exhaustive exercise stimulated FR increase,and prompted the occurrence of fatigue.The mechanism is likely found in the following points:(1)The activity of creatine kinase decreased and resulted in the increase of adenosine monophosphate (AMP).Under the catalysis of adenine trans-aminase,AMP converted to hypoxanthine nucleotide(IMP),IMP converted to hypoxanthine catalysis by 5-nucleotidase and nucleosidase and by catalysis of hypoxanthine oxidase,hypoxanthine produced a mass of superoxide anion.19This set of reactions can cause a signi ficant increase of lipid peroxidation levels and damage the skeletal muscle cell.(2)The FR metabolism was enhanced when the rats exercised.FR also likely caused lipid peroxidation,which damaged the cell membrane and possibly also caused gene mutation,which further led to synthesis error of protein and enzyme.Finally,the activities of many enzymes (such as phosphofructokinase,citroyl synthetase,isocitrate dehydrogenase,and oxoglutarate dehydrogenase complex) associated with energy metabolism are decreased causing reduced metabolism.Lipid peroxidation can cause a decrease of membrane fluidity and a change of membrane hydromechanics,making the membrane more fragile and may cause calcium overload.20As a result,ATP synthesis decreased.(3)The change of structure of membrane lipid led to the pore of myolemma and mitochondrial membrane enlargement and a change of membrane permeability.This change will likely result in membrane injury,and thus the structures of subcellular organelle are also damaged and led to a series of disorders.21(4)In the process of biological membrane lipid peroxidation,the metabolic pathways of arachidonic acid are activated and many highly active substances such as leukotrienes and prostaglandinare produced.Meanwhile the oxygen FR generated can cause lipid peroxidation and again can create a vicious cycle and further increase the skeletal muscle cell injury.22

    As compared with the NTSNE group,the activity of T-AOC increased(p<0.05)while MDA level decreased(p<0.01),and the activity of SOD,GSH-Px,GR,and GSH showed a tendency of increase(but no statistical difference)in TSNE group.The results of the present experiment indicate thatTS has the capacity of scavenging FR even in the rest state.The antioxidative effect of TS may relate to its chemical structure.Considering TS structure,the parent nuclear is rich in phenolic hydroxyl and can combine with FR and form a stable semiquinone.Thus,TS can break the chain reaction of FR.The enol and keto structure of TS can also capture and directly clear FR.Yoshiki et al.23studied the TS of DDMP group by molecular orbital method and found the antioxidative activity of DDMP saponins related to C-6 and reported that reactive oxygen species were eliminated mainly in this area.Lee et al.24reported that TS also has a strong total antioxidant capacity and anti-active oxygen capacity in vitro.TS can inhibit lipid peroxidation in liver tissue and alleviate the swelling of liver mitochondria,inhibit erythrocyte membrane lipid peroxidation,and reduce hemolysis of the red blood cells.

    Typically,the body keeps a dynamic balance between the generation and the removal of FR.However,under the condition of exhausted exercise,FR in the body can increase signi ficantly.25,26SOD,CAT,and GSH-Px are common antioxidative enzymes which can eliminate FR and reduce this imbalance.Under normal physiological conditions and appropriate exercise work rates,the antioxidant enzymes system of the body,by the way of their respective roles,can maintain a dynamic balance between the FR generation and removal(Fig.3).

    Data found in Table 1 show that the T-AOC in the TSNE group was signi ficantly higher than that in the other groups. This difference maybe in part due to TS eliminating FR and reducing the consumption of non-enzyme substance such as vitamin C,vitamin E,GSH,and cysteine.Thereby,the T-AOC of quadriceps femoris was increased signi ficantly.According to the present experimental data,it can be inferred that the increase of T-AOC in quadriceps femoris of rats is mainly due to the increase of the non-enzyme system.

    Fig.3.The scavenging effect of antioxidant enzymes on free radicals in vivo. CAT=catalase;GR=glutathione reductase;GSH=reduced glutathione; GSH–Px=glutathione peroxidase;GSSG=oxidized glutathione;NADP= oxidized nicotinamide adenine dinucleotide phosphate;NADPH=reduced nicotinamide adenine dinucleotide phosphate;SOD=superoxide dismutase.

    Data presented in Table 2 showed that TS decreased the activity of serum LDH and BUN while increasing blood testosterone level whether the rats were in a rested or an exhausted exercise state.There are 5 kinds of blood LDH isozyme.LDH1mainly exists in cardiac muscle tissue;LDH5mainly exists in skeletal muscle.The present study evaluated total LDH,which re flects the permeability and the damage extent of the sarcolemma.The results show that NTSE group led to an increase of LDH activities,which suggest increased sarcolemma permeability.This increased permeability may be due to myotasis or FR injury.TS intervention decreased LDH activity,which supports that TS provides skeletal muscle cell protection that is likely achieved through an antioxidative effect.

    TheserumBUNlevelcanre flectkidneyfunctionandprotein metabolism.Although protein can supply energy in exercise, protein’smainbiologicalfunctionsareprotectionandreparation of the body,not supplying energy.When the body is short of glycogen,exercisedathighintensitiesandforlongtimeperiods, protein metabolism can rise and serum BUN will increase.The experiment completed here and the data presented show that exhaustiveexerciseledtoanincreaseofserumBUNlevelwhich indicates increased protein metabolism.TS intervention signi ficantly decreased BUN level and a statistical difference among groups was found.This difference supports the contention that TS can provide protection from protein used by the body andTS may be an essential factor in the explanation for the increased exercise time to exhaustion reported in this study.

    As seen from the data presented in Table 2,TS signi ficantly increased rat serum testosterone level.As compared with the NTSNE group,the serum testosterone level sharply increased in the TSNE group(p<0.01).This increase shows that TS can stimulate testosterone secretion and increase testosterone level in the rat.The SD of testosterone data in the TSNE group was larger than in other groups,indicating a higher dispersion degree for the testosterone data and a higher individual variation for this group.The reason for this greater degree of dispersion is not clear,but it may be caused in part by some random errors in the testing process,which could affect objectivity of the results.By the intervention of TS,compared with NTSE group,the serum testosterone increased in the TSE group,but this difference was not statistically signi ficant.As the main androgen and anabolic steroid,testosterone can sustain muscle volume and muscle weight,sustain bone density and strength,and improve stamina.Testosterone can also affect hematopoiesis,calcium balance,bone mineralization,lipid metabolism,and glycometabolism.That TS can increase the exercise time to exhaustion in the rat is likely related to TS ability to stimulate the secretion of testosterone.This question is still in need of further study to better understand the proper mechanism responsible.

    5.Conclusion

    According to the data analysis and discussions of the experimental results presented in this study,the following conclusions were drawn:(1)TS can signi ficantly increase the rats’exercise time to exhaustion,and TS has a positive effect on preventing fatigue;(2)TS can signi ficantly increase the serum testosterone level and decrease serum LDH and BUN activity;these changes indicate that TS can provide protection of protein use,sustain moderate permeability of the sarcolemma,andstimulate synthesis and secretion of testosterone;(3)TS positively affected antioxidative capability of the rats’quadriceps femoris,especially by improved T-AOC and decreased MDA. The antioxidative effect of TS in the quadriceps femoris of exercised rats is likely dependent on the non-enzyme system.

    Acknowledgment

    This study was supported by the National Natural Science Foundation of China(No.11101354).

    Authors’contributions

    ZGL designed the study and drafted the paper;RXN,YL, ZHL,and ZYX performed the experiments;CXY participated in the data analysis and drafted the manuscript.All authors have read and approved the final version of the manuscript,and agree with the order of presentation of the authors.

    Competing interests

    The authors declare that they have no competing interests.

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    25 December 2014;revised 2 June 2015;accepted 9 October 2015

    Available online 22 January 2016

    Peer review under responsibility of Shanghai University of Sport.

    *Corresponding author.

    E-mail address:xbaili@126.com(Z.Liu)

    http://dx.doi.org/10.1016/j.jshs.2016.01.016

    2095-2546/?2017 Production and hosting by Elsevier B.V.on behalf of Shanghai University of Sport.This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).

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