劉新倫,王超,牛麗華,劉志立,張錄德,陳春環(huán),張榮琦,張宏, 王長(zhǎng)有,王亞娟,田增榮,吉萬全
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普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種抗赤霉病基因的分子鑒別
劉新倫1,王超1,牛麗華1,劉志立2,張錄德3,陳春環(huán)1,張榮琦1,張宏1, 王長(zhǎng)有1,王亞娟1,田增榮1,吉萬全1
(1西北農(nóng)林科技大學(xué)農(nóng)學(xué)院/旱區(qū)作物逆境生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室,陜西楊凌712100;2陜西省城固縣種子管理站,陜西漢中 723200;3陜西省扶貧開發(fā)辦公室,西安 710006)
赤霉?。‵usarium head blight,F(xiàn)HB)是世界范圍內(nèi)嚴(yán)重危害小麥生產(chǎn)的病害之一。十倍體長(zhǎng)穗偃麥草()具有優(yōu)良的赤霉病抗性,普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種西農(nóng)509、西農(nóng)511和西農(nóng)529在田間展現(xiàn)出較強(qiáng)的赤霉病抗性。本文旨在對(duì)這3個(gè)品種的赤霉病抗性基因進(jìn)行分子鑒別,為它們?cè)谛←湷嗝共】剐赃z傳改良中的應(yīng)用提供理論依據(jù)。通過赤霉病菌()人工接種鑒定,明確3個(gè)小麥新品種對(duì)赤霉病的抗性水平。利用偃麥草E組染色體(臂)第1—7同源群的特異引物對(duì)3個(gè)普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種及其主要親本小偃693、小偃597和十倍體長(zhǎng)穗偃麥草進(jìn)行分子鑒定,確定其長(zhǎng)穗偃麥草的遺傳區(qū)段。利用與長(zhǎng)穗偃麥草7EL染色體上抗赤霉病基因緊密連鎖的標(biāo)記對(duì)實(shí)驗(yàn)材料進(jìn)行分析,明確該抗性基因與的關(guān)系。鑒定結(jié)果表明,西農(nóng)509、西農(nóng)529和西農(nóng)511的赤霉病抗性與中抗對(duì)照品種揚(yáng)麥158的抗性水平相當(dāng),表現(xiàn)為中抗。105個(gè)長(zhǎng)穗偃麥草E基因組特異標(biāo)記中有7個(gè)在3個(gè)新品種中均能擴(kuò)增出長(zhǎng)穗偃麥草的特異條帶,其中5個(gè)標(biāo)記定位于7EL染色體臂上,2個(gè)標(biāo)記定位于7ES染色體臂上。利用97個(gè)定位于7E染色體的特異標(biāo)記進(jìn)一步對(duì)小偃693、小偃597和3個(gè)新品種的遺傳片段進(jìn)行鑒別,結(jié)果表明20個(gè)標(biāo)記能在5個(gè)長(zhǎng)穗偃麥草衍生品種(系)擴(kuò)增出穩(wěn)定的十倍體長(zhǎng)穗偃麥草的特異條帶,其中包括與緊密連鎖的和等6個(gè)標(biāo)記(7EL 149.00—7EL 153.77),即表明該區(qū)段源自于十倍體長(zhǎng)穗偃麥草,跨距約89 cM。然而,已報(bào)道的7EL染色體臂末端與兩側(cè)緊密連鎖的分子標(biāo)記(7EL 153.77)(7EL 154.70)、(7EL 156.27)、(7EL 158.02)、(7EL 158.97)和(7EL 160.00)等在3個(gè)新品種及小偃597中均沒檢出長(zhǎng)穗偃麥草的特異條帶。普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種西農(nóng)509、西農(nóng)511和西農(nóng)529具有較好的赤霉病抗性,攜帶來自于十倍體長(zhǎng)穗偃麥草7E染色體的遺傳區(qū)段,然而該抗赤霉病基因不同于。
普通小麥;十倍體長(zhǎng)穗偃麥;赤霉??;;分子標(biāo)記
【研究意義】小麥赤霉?。‵usarium head blight,F(xiàn)hb)是由禾谷鐮孢()引起的一種小麥重要穗部病害,主要發(fā)生于潮濕及半潮濕地區(qū)[1]。在小麥赤霉病大流行年份,可以造成70%,甚至是100%的產(chǎn)量損失[2]。小麥赤霉病的發(fā)生不僅造成嚴(yán)重的產(chǎn)量損失,而且感病的小麥籽粒還含有各種毒素(尤其是DON毒素),嚴(yán)重威脅人和動(dòng)物[3-4]的食品安全。近年來,隨著全球氣候變暖以及耕作制度的變化,小麥赤霉病逐漸由偶發(fā)病害上升為常發(fā)病害,發(fā)生面積也不斷擴(kuò)大。在中國(guó),赤霉病已由常發(fā)的長(zhǎng)江中下游麥區(qū)擴(kuò)展到黃淮麥區(qū)和華北麥區(qū)[5-6]。目前還沒有徹底防治小麥赤霉病的化學(xué)藥劑,最為經(jīng)濟(jì)環(huán)保的控制小麥赤霉病危害的途徑就是培育和選用抗赤霉病品種。對(duì)普通小麥-長(zhǎng)穗偃麥草()衍生小麥新品種進(jìn)行赤霉病抗性鑒定和抗性基因的分子鑒別,可為深入研究和應(yīng)用長(zhǎng)穗偃麥草的赤霉病抗性基因提供理論依據(jù),對(duì)于小麥赤霉病抗性育種具有重要意義?!厩叭搜芯窟M(jìn)展】赤霉病抗源相對(duì)較少,國(guó)內(nèi)外學(xué)者通過大量的基因資源研究,迄今從普通小麥及其近緣種屬中僅鑒定出7個(gè)赤霉病抗性主效基因,分別是來自蘇麥3號(hào)的[7]和[8]、大賴草的[9]、望水白的[10]和[11]、披堿草屬的[12]和十倍體長(zhǎng)穗偃麥草的[13]。中國(guó)工作者經(jīng)多年努力,培育出了揚(yáng)麥系列、寧麥系列和鄂麥系列等優(yōu)良抗性品種,但大面積生產(chǎn)中缺乏抗赤霉病的高產(chǎn)小麥品種[14]??v觀國(guó)內(nèi)數(shù)十年的小麥抗赤霉病育種,其抗源仍然主要局限于蘇麥3號(hào)、望水白和它們的衍生系。赤霉病抗源基礎(chǔ)狹窄是小麥育種工作取得突破性進(jìn)展的瓶頸,發(fā)掘新的小麥赤霉病抗源成為提高小麥赤霉病抗性的關(guān)鍵[15]。由此可見,鑒定和利用小麥近緣種屬的赤霉病抗性基因尤為重要[16]。長(zhǎng)穗偃麥草是小麥的近緣植物,其E基因組含有許多優(yōu)異的抗性基因,如抗旱、抗寒能力很強(qiáng),對(duì)白粉病[17]和銹病完全免疫(7E染色體)[18],高抗條紋花葉病和小麥黃矮?。?E、7E染色體)[19-20],對(duì)赤霉病表現(xiàn)較強(qiáng)的耐病性(1E、7E染色體)[18,21-23],且又能與小麥進(jìn)行遠(yuǎn)緣雜交,實(shí)現(xiàn)抗病基因的遺傳轉(zhuǎn)移。因此,長(zhǎng)穗偃麥草是小麥抗病育種的重要抗源親本之一[24]。八倍體小偃麥小偃693和小偃7430染色體總數(shù)均為56條,但是它們分別含有8對(duì)和6對(duì)長(zhǎng)穗偃麥草染色體,還分別含有4條和2條小麥-長(zhǎng)穗偃麥草易位染色體[25]。小偃7430與普通小麥雜交、回交選育而成了許多抗白粉病和條銹病、稈銹病的小偃麥衍生新品種(系)[26-28]。然而,目前關(guān)于小偃693衍生后代抗病性的研究鮮有報(bào)道。小偃597是利用小偃693與普通小麥雜交、回交選育成功的含有十倍體長(zhǎng)穗偃麥草遺傳物質(zhì)的普通小麥品種,對(duì)赤霉病表現(xiàn)抗性[29]。研究表明,長(zhǎng)穗偃麥草中已發(fā)現(xiàn)3個(gè)赤霉病抗性主效基因,分別定位于二倍體長(zhǎng)穗偃麥草1E染色體[30-32]和7E染色體[22,32],十倍體長(zhǎng)穗偃麥草7E染色體[13,18,23]。其中十倍體長(zhǎng)穗偃麥草7E染色體長(zhǎng)臂上赤霉病抗性基因被正式命名為[13],可解釋抗赤霉病表型變異的30.46%[18]。Guo等[13]將定位在7EL染色體末端1.7 cM范圍內(nèi),且兩側(cè)緊密連鎖的分子標(biāo)記分別為和;該抗病區(qū)間對(duì)應(yīng)于水稻、短柄草和高粱的基因組大小分別為18.8、37.1和27.0 kb,并開發(fā)出與緊密連鎖的特異分子標(biāo)記,如等,這些分子標(biāo)記可用于該基因的分子標(biāo)記輔助選擇?!颈狙芯壳腥朦c(diǎn)】西農(nóng)509、西農(nóng)511和西農(nóng)529是由西北農(nóng)林科技大學(xué)農(nóng)學(xué)院培育出的含有十倍體長(zhǎng)穗偃麥草遺傳物質(zhì)的高產(chǎn)、優(yōu)質(zhì)、抗病的普通小麥新品種[33-34],田間赤霉病表現(xiàn)輕[6,33-34]。這些品種已在黃淮冬麥區(qū)南片的河南?。喜康静琨渽^(qū)除外)、安徽省沿淮及淮北地區(qū)、江蘇省淮北地區(qū)和陜西省關(guān)中地區(qū)示范推廣種植。2014—2016年在河南南陽和駐馬店、江蘇省徐州和安徽淮北生態(tài)區(qū)域等赤霉病重發(fā)區(qū),這些品種赤霉病輕度發(fā)生,發(fā)病率僅為1%—3%,屬于中抗或中感抗性水平[6,35-37]。然而,目前它們赤霉病的抗性遺傳基礎(chǔ)并不清楚。【擬解決的關(guān)鍵問題】對(duì)3個(gè)衍生新品種進(jìn)行赤霉病抗性進(jìn)行人工接種鑒定,然后用偃麥草E組染色體特異標(biāo)記對(duì)西農(nóng)509、西農(nóng)511和西農(nóng)529及其主要親本小偃693、小偃597和十倍體長(zhǎng)穗偃麥草進(jìn)行分子鑒定,初步確定其赤霉病抗性基因的來源,在此基礎(chǔ)上利用長(zhǎng)穗偃麥草7EL染色體上的特異分子標(biāo)記及與抗性基因緊密連鎖的分子標(biāo)記對(duì)實(shí)驗(yàn)材料進(jìn)一步進(jìn)行分子鑒別,為這些抗赤霉病的長(zhǎng)穗偃麥草衍生新品種的推廣和利用提供理論依據(jù)。
普通小麥中國(guó)春(),十倍體長(zhǎng)穗偃麥草;普通小麥-十倍體長(zhǎng)穗偃麥草遠(yuǎn)緣雜交后代材料和品種:八倍體小偃麥小偃693(染色體組成為AABBDDEE)和普通小麥品種小偃597;新培育的普通小麥-十倍體長(zhǎng)穗偃麥草衍生品種西農(nóng)509、西農(nóng)511和西農(nóng)529。赤霉病抗性鑒定的對(duì)照品種蘇麥3號(hào)、揚(yáng)麥158、寧麥9號(hào)和安農(nóng)8455。小偃693是本研究中3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生新品種的間接親本,普通小麥品種小偃597是西農(nóng)529和陜麥159的直接親本,是西農(nóng)511的間接親本(圖1)。
圖1 普通小麥-長(zhǎng)穗偃麥草衍生小麥新品種長(zhǎng)穗偃麥草遺傳基因溯源
抗赤霉病人工接種鑒定由江蘇省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所在南京鑒定完成,赤霉菌菌株為F0301、F0609、F0980和F1126,鑒定方法和調(diào)查記載標(biāo)準(zhǔn)參見“小麥抗病蟲性評(píng)價(jià)技術(shù)規(guī)范”系列標(biāo)準(zhǔn)(NY/T 1443—2007)[38]。蘇麥3號(hào)、揚(yáng)麥158和寧麥9號(hào)為抗病對(duì)照品種,安農(nóng)8455為感病對(duì)照品種。抗性分級(jí)標(biāo)準(zhǔn):抗(R),平均嚴(yán)重度<1.5(蘇麥3號(hào)的平均反應(yīng)級(jí)+標(biāo)準(zhǔn)差);中抗(MR),1.5≤平均嚴(yán)重度<2.5(揚(yáng)麥158的平均反應(yīng)級(jí)+標(biāo)準(zhǔn)差);中感(MS),2.5≤平均嚴(yán)重度<3.5,感(S),平均嚴(yán)重度≥3.5。
小麥幼葉基因組DNA采用微量CTAB法 提取,干燥后溶于TE緩沖液。由于長(zhǎng)穗偃麥草中已發(fā)現(xiàn)的赤霉病抗性主效基因均被定位于E基因組[13,18,21-23,30-32],本研究選用長(zhǎng)穗偃麥草E組染色體特異分子標(biāo)記[22,39-47]及7EL上連鎖的分子標(biāo)記[13,18,48-49]對(duì)參試材料進(jìn)行長(zhǎng)穗偃麥草遺傳物質(zhì)和的檢測(cè)(附表1)。引物由生物工程(上海)有限公司合成。PCR擴(kuò)增反應(yīng)在GeneAmp 9700型熱循環(huán)儀(ABI,USA)上進(jìn)行,反應(yīng)混合液總體積為10 μL,其中包括0.25 U酶、1.0 μL 10×Buffer、1.5 mmol·L-1MgCl2、0.2 mmol·L-1dNTPs、0.5 μmol·L-1primer(上下游引物)、50—100 ng模板DNA。擴(kuò)增程序:94℃ 2 min;94℃ 1 min,50—60℃ 1 min,72℃ 1 min,35個(gè)循環(huán);72℃ 5 min。PCR產(chǎn)物用8.0%的非變性聚丙烯酰胺凝膠上電泳檢測(cè),銀染后照相觀察并記錄。
江蘇省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所先后對(duì)西農(nóng)509(陜麥509)(2008—2010)、西農(nóng)529(2013—2015)和西農(nóng)511(2015—2016)進(jìn)行了赤霉病抗性人工接種鑒定。由表1調(diào)查結(jié)果可以看出,西農(nóng)509、西農(nóng)529和西農(nóng)511的赤霉病鑒定結(jié)果均與中抗對(duì)照品種揚(yáng)麥158的抗性水平相當(dāng),表現(xiàn)為中抗。
用105個(gè)長(zhǎng)穗偃麥草E基因組的特異標(biāo)記(其中1E、2E、3E、4E、5E、6E和7E染色體上特異標(biāo)記分別有29、12、10、5、14、10和25個(gè))分別對(duì)中國(guó)春、十倍體長(zhǎng)穗偃麥草、西農(nóng)509、西農(nóng)511和西農(nóng)529進(jìn)行分子檢測(cè)。結(jié)果顯示,71個(gè)標(biāo)記在中國(guó)春和十倍體長(zhǎng)穗偃麥草間有多態(tài)性,但只有(圖2-A)、、、、(圖2-B)和等7個(gè)標(biāo)記在3個(gè)普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種中均擴(kuò)增出長(zhǎng)穗草的特異產(chǎn)物,其中前5個(gè)標(biāo)記都位于7E長(zhǎng)臂,和則位于7E短臂。E基因組分子標(biāo)記分析結(jié)果表明,西農(nóng)509、西農(nóng)511和西農(nóng)529攜帶來自于十倍體長(zhǎng)穗偃麥草7E染色體的遺傳物質(zhì)。
用定位于7E染色體長(zhǎng)、短臂上的75和22個(gè)分子標(biāo)記(包括DArT標(biāo)記、SSR標(biāo)記、EST標(biāo)記)[13,36,43,45,48-49]分別對(duì)中國(guó)春、十倍體長(zhǎng)穗偃麥草、3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生新品種及它們重要的親本小偃693和小偃597進(jìn)行分子標(biāo)記分析。結(jié)果表明,7ES 64.63到7EL 153.27長(zhǎng)約89 cM之間共有20對(duì)引物在小偃693、小偃597和3個(gè)新品種中均能擴(kuò)增出穩(wěn)定的長(zhǎng)穗偃麥草的特異條帶,其中12個(gè)引物定位于7E長(zhǎng)臂,8個(gè)定位于7E短臂(表2)。由此進(jìn)一步推斷西農(nóng)509、西農(nóng)511和西農(nóng)529這3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生新品種含有十倍體長(zhǎng)穗偃麥草7E染色體跨著絲粒區(qū)域的中段遺傳物質(zhì)。
用定位于7EL 140.03到160.00處的與緊密連鎖的40個(gè)系列標(biāo)記、7個(gè)系列標(biāo)記、4個(gè)SSR標(biāo)記(和)[13,36,43,45]分別對(duì)中國(guó)春、十倍體長(zhǎng)穗偃麥草、3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生新品種以及赤霉病抗性基因的供體親本小偃693和小偃597進(jìn)行基因標(biāo)記分型分析。結(jié)果表明(表2),(圖2-C)(7EL 149.51)、(圖2-D)、(圖2-E)、和(7EL 153.27)(圖2-A)等6個(gè)標(biāo)記在3個(gè)新品種及小偃693和小偃597兩親本中具有十倍體長(zhǎng)穗偃麥草的特異性條帶;(7EL 153.77)、(7EL 154.70)、(7EL 156.27)(圖2-F)和(7EL 158.02)(圖2-G)4個(gè)標(biāo)記能在八倍體小偃麥小偃693中擴(kuò)增產(chǎn)生十倍體長(zhǎng)穗偃麥草的特異性條帶,而在小偃597和3個(gè)小麥新品種中沒有十倍體長(zhǎng)穗偃麥草的特異條帶;(7EL 158.97)和(7EL 160.00)(圖2-H)在小偃693、小偃597和3個(gè)小麥新品種中均沒有十倍體穗偃麥草的特異條帶。
表1 3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生小麥新品種的赤霉病病情指數(shù)和抗性評(píng)價(jià)
*本試驗(yàn)數(shù)據(jù)來源于小麥國(guó)家區(qū)域試驗(yàn)赤霉病抗性鑒定結(jié)果 The data of this table were derived from the results of wheat regional resistance test for Fusarium head blight。R:抗病resistant;MR:中抗moderately resistant;S:感病Susceptible;HS:高感Highly susceptible
表2 7E染色體上部分分子標(biāo)記對(duì)3個(gè)小麥新品種及其主要親本的擴(kuò)增片段大小
M:DEL2000;1:中國(guó)春Chinese Spring (CS);2:十倍體長(zhǎng)穗偃麥草Th. ponticum;3:小偃693 Xiaoyan 693;4:小偃597 Xiaoyan 597;5:西農(nóng)509 Xinong 509;6:西農(nóng)511 Xinong 511;7:西農(nóng)529 Xinong 529。左側(cè)為分子量標(biāo)記條帯大?。╞p)。箭頭所示為偃麥草染色體的特異條帶The numbers at the top of the gels are lane numbers. The numbers on each photo is the marker bands size in bp. Arrows indicated the Thinopyrum specific bands linked toFhb7
在抗赤霉病育種方面,自20世紀(jì)70年代,中國(guó)研究者從普通小麥及其近緣物種3萬多份材料中篩選出蘇麥3號(hào)和望水白兩個(gè)抗性強(qiáng)而穩(wěn)定的普通小麥品種,但其農(nóng)藝性狀較差[50],應(yīng)用過程中赤霉病抗性很難和豐產(chǎn)性結(jié)合起來,生產(chǎn)上亟需高產(chǎn)抗赤霉病的小麥品種。然而,自揚(yáng)麥158后,大面積推廣的品種均未解決好赤霉病抗性與豐產(chǎn)性負(fù)相關(guān)的問題[14]。因而開拓新的抗源以及如何高效利用小麥近緣種的抗病基因已成為小麥抗赤霉病育種亟待解決的重要問題[51]。
長(zhǎng)穗偃麥草是小麥遠(yuǎn)緣雜交育種的重要親本材料之一,研究表明其1E和7EL染色體上攜帶有抗赤霉病基因[13,18,21-23,26]。Jauhar等[31]將抗赤霉病的長(zhǎng)穗偃麥草與硬粒小麥雜交,獲得了赤霉病抗性提高顯著的衍生系。張璐璐等[52]利用偃麥草7E染色體特異分子標(biāo)記篩選結(jié)合GISH驗(yàn)證培育了小麥-長(zhǎng)穗偃麥草7EL異位系和7ES附加系,赤霉病抗性鑒定表明7EL易位系的赤霉病抗性明顯較好。西北農(nóng)林科技大學(xué)培育的普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種西農(nóng)509、西農(nóng)511和西農(nóng)529目前已成為黃淮冬麥區(qū)南片和陜西省關(guān)中地區(qū)示范推廣的高產(chǎn)優(yōu)質(zhì)的小麥新品種。據(jù)報(bào)道,上述3個(gè)小麥新品種在河南、安徽、江蘇等赤霉病重發(fā)地田間表現(xiàn)赤霉病很輕[6,31-33]。本研究中,通過人工抗性鑒定,這3個(gè)品種的赤霉病抗性與揚(yáng)麥158相當(dāng),屬于中抗水平,且抗性穩(wěn)定。西農(nóng)509、西農(nóng)511和西農(nóng)529赤霉病輕且綜合性狀和豐產(chǎn)性好,有望在黃淮冬麥區(qū)南片赤霉病常發(fā)區(qū)和重發(fā)區(qū)大面積推廣種植。
本研究利用長(zhǎng)穗偃麥草E基因組特異的引物初步確定3個(gè)小麥新品種的長(zhǎng)穗偃麥草的遺傳物質(zhì)來自7E染色體跨著絲粒區(qū)域的中段,說明其抗赤霉病基因來源于7E染色體,研究結(jié)果與前人的研究一致[13,18,22-23,52]。來自十倍體長(zhǎng)穗偃麥草的抗赤霉病基因已定位于7EL染色體上,(7EL 156.27)和(7EL 158.02)分別與其緊鄰[13]。為了探究上述3個(gè)小麥新品種中赤霉病抗性基因與的關(guān)系,選用偃麥草7EL染色體(7EL 140.03—7EL 160.00)上與連鎖的分子標(biāo)記對(duì)這3個(gè)新品種及其主要親本小偃693、小偃597和十倍體長(zhǎng)穗偃麥草進(jìn)一步進(jìn)行分子標(biāo)記分析。結(jié)果發(fā)現(xiàn),7EL上6個(gè)與緊密連鎖的分子標(biāo)記在3個(gè)新品種中擴(kuò)增產(chǎn)生長(zhǎng)穗偃麥草的特異產(chǎn)物,其中,和(7EL 153.27)3個(gè)標(biāo)記與的遺傳距離僅3.0—4.0 cM。Guo等[13]利用與抗赤霉病基因緊密連鎖的分子標(biāo)記(和)進(jìn)行分子輔助選擇,從濟(jì)麥22與小麥-長(zhǎng)穗偃麥草抗赤霉病短片段易位系SDAUl881雜交、回交后代中成功獲得2個(gè)新的改良系SDAU2003和SDAU2028。然在本研究中,與緊鄰的4個(gè)標(biāo)記(7EL 153.27)、(7EL 154.70)、(7EL 156.27)和(7EL 158.02),在小偃597和3個(gè)長(zhǎng)穗偃麥草衍生小麥新品種中均沒有擴(kuò)增出長(zhǎng)穗偃麥草的特異性條帶,只在八倍體小偃麥小偃693中有長(zhǎng)穗偃麥草的特異性條帶。同時(shí),7EL末端的標(biāo)記(7EL 158.97)和(7EL 160.00)不僅在3個(gè)小麥新品種中沒有偃麥草特異條帶,而且在小偃693和小偃597中也沒有。何方[25]以十倍體長(zhǎng)穗偃麥草總基因組DNA為探針對(duì)小偃693根尖細(xì)胞染色體進(jìn)行GISH分析,發(fā)現(xiàn)小偃693中不僅含有4對(duì)Ee組染色體和4對(duì)Eb組染色體,同時(shí)還含有4條小麥-長(zhǎng)穗偃麥草異位染色體。本研究中,西農(nóng)509、西農(nóng)511和西農(nóng)529 這3個(gè)普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種中含有長(zhǎng)穗偃麥草7E染色體跨著絲粒區(qū)域的中段的遺傳物質(zhì),但缺失了相關(guān)聯(lián)的7EL末端片段,究其原因,也許是在八倍體小偃麥小偃693與普通小麥雜交、回交選育過程中,7E染色體與普通小麥染色體發(fā)生了異位,導(dǎo)致7EL末端片段丟失,這個(gè)推測(cè)有待借助GISH和FISH等技術(shù)進(jìn)一步驗(yàn)證。
西農(nóng)509、西農(nóng)511和西農(nóng)529等3個(gè)普通小麥-十倍體長(zhǎng)穗偃麥草衍生新品種在黃淮南片麥區(qū)赤霉病重發(fā)地區(qū)表現(xiàn)赤霉病很輕,同時(shí)人工接種鑒定表現(xiàn)為中抗赤霉病。赤霉病抗性基因分子鑒定表明,長(zhǎng)穗偃麥草7ES 64.63—7EL 153.27之間有20對(duì)引物在3個(gè)新品種和它們的主要親本小偃693和小偃597中均擴(kuò)增出穩(wěn)定清晰的長(zhǎng)穗偃麥草特異條帶,然而在這3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生新品種及小偃597中沒有檢測(cè)到7EL末端抗赤霉病主效QTL的分子標(biāo)記產(chǎn)物。推測(cè)這3個(gè)普通小麥-長(zhǎng)穗偃麥草衍生新品種含有來自于十倍體長(zhǎng)穗偃麥草的不同于的抗赤霉病基因。
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(責(zé)任編輯 岳梅)
附表1 所利用的長(zhǎng)穗偃麥特異分子標(biāo)記
Table s1 Specific molecular markers ofand their sequences used in this study
標(biāo)記名稱Marker name引物序列Nucleotides sequence of markers所在染色體genome location擴(kuò)增片段大小Fragment size (bp)參考文獻(xiàn)Reference左引物L(fēng)eft primer (5′-3′)右引物Right primer (5′-3′) 1Ee STS241TAACTAGCACACCAAATCGTTTTTGCAAACAATTCATTGGGGTGGGTAAAGGAAGTGG1E250[41] 1Ee STS318GAATTCATCATAAAAACAGATCAAAGGTAACGTAAACTTCCCGTCTGCTAC1E280[41] SP1E_No.1 TATCAAATAATATGTGCATG CATGACAGAGATATGAAAAG 1E276[42] SP1E_No.21 CATCATATAAGAATGCAGGA TCAACAGCACGCCAAC 1E230[42] SP1E_No.23 TAGGAAAGAAATCTGAAATA TGAGACAGCAGCAAAA 1E220[42] SP1E_No.27 GGCTGATGCACTGACGT TCCGAAATCTGAAAAGGAG 1E273[42] SP1E_No.29 ACTGTTTTTTTTTTCGAG AGCGCACAAGATGAGAC 1E151[42] SP1E_No.30 TTAGAAAAATTCAACCCG AATCTTGAAACATGGGAG 1E80[42] SP1E_No.31 CCGGTTTGAAAGAAGAGA CAACAAGCAAATGAGGGA 1E158[42] SP1E_No.33 TCACCTATGAGATCGCT TATCCGAAGAACCTGTAC 1E285, 175[42] SP1E_No.34 TGTTCCTTGCATCATCT AACTCACCTATCGGTCTAT 1E158[42] SP1E_No.35 TTGACAACCCAATCTG TCACTGATAAATAACAAGAG 1E162[42] SP1E_No.36 TGACGGCAACGGAGTG TCTGCCGAACATGGAATC 1E150[42] SP1E_No.37 AGCACTTGGGTTTTCAT GGATTGGAGGGTCACTA 1E260[42] SP1E_No.38 TTCAAGAAGTATAGCTCGTT AACCATAAAGTCCCAACA 1E300[42] SP1E_No.41 GGAAAGGGCACTCCAACC GCGTCACATCCTGGTTCG 1E210[42] SP1E_No.42 CTCCATCCCTCCACTT AACATCAGCAGTTGGG 1E190[42] SP1E_No.44 TTGACTTCTCCCAAGTTG AATGGAAAGGAAAATCTG 1E203[42] SP1E_No.45 TCTATTTTGCTATTTGCC TTTTGTCTTCATCACCAT 1E568[42] SP1E_No.47 TACTCGCTACTTACAATCT GAACATTATTGTGAAGCA 1E255[42] SP1E_No.48 CGGAATGTCCTCACA ATTGGCTAATGCTATGA 1E280[42] SP1E_No.8 AATATTTATGGCGCACGCAG TCATCAGGGGAGTGAGGAGC 1E400, 200[42] Xedm17TAGCTGCATCTCTGGTTTGGCTAGTTGTTGTTGCTGCGAAA1E396[39] Xedm74GTAAGTCGACCGAGGAGACGCCTTCTTGCTTGGCATTCTC1EL360[39] Xedm92ATCCGCGACGACAATGTAAGCTGGTGTGGATCTTGACGTG1EL185[39] Xmag2137 GAGTTCGATGACATGGCTGACCGATAACAAAGTGCGTGAA1E580, 250[43-44] Xmag3489CGATGTAAAGACTGTAGCCTGTAGGCGTCCATTTGGTTCGGTGAG1E330[43-44] Xmag834 ACGCCCTCAAACAAGAAAGACTTCCTCTCCTTCCCCAGAG1E500[43-44] Z34-1E200GATGTCACCAAGCCCTACTCCTACGAAACACCCAAG1E225[47] 2Ee STS116TTAACCAGGGATACAAAAACACCATAATGAATTCAATTACCTTGTGGATGCAAACAC2E150[41] 2Ee STS182AATTCATTTCTAATAGATACTCGAATGAATTAACTGAGGAGAGGCTACAACATCC2E182[41] SM2E-1CAGGTCCAAAAAACCAGCC GAATTCGAGGTTCCGCAG 2E480, 210[40] SM2E-3GTGTGTGCGCATGAACTACTT GGAGTCGTTGGTTGGACATG 2E225[40] SM2E-5ATGCTTTTCCCTTCCAACAG CAAACGAAAAGCTGACCAAAC 2E173[40] Xmag2090 AAAGGTGTAAGCGTGGCATCACACGCATAATGCACTCCAG2E300, 255[43-44] Xedm28GCTCACTCACGCATCATAGCGTTGGCGGAATCCTTCTTC2ES & 3EL150[39] Xedm8GACCCCCACTTCCTGCTCGGGCAAAACTGTGGCTATTG2ES & 3EL145[39] Xedm96TCCAAGACTGTGCCTCAAGAGCGTTGAACTCCTGTTCCAT2ES & 3ESL175[39] Xmag3253 CTGCTGCTTGGGATCATTCTGCTGGTGAGAGTTGGAAACC2E600, 310[43-44] Xmag3616 GTCCGTGCCATTCAACTTCTTCTATCCACTCCATTCCAAGC2E&3E260, 200[43-44] Xmag4137 CTCGTGTGGCTCATCAATGTTGACATCTGGTTGGTGGTCA2E&3E490[43-44] iPBS8TGCAGGTCGACGAGCGGGTATGCTTTG3E428[46] SM3E-1CACACGCAATTCAACAACTCAAG ACGAATTGACCGTCGATCAGA 3E195[40] SM3E-2GCAGCAGAGAACGTACACTTGTATGGCAAAAGTATTCTGAACCGT3E154[40] TNAC1248ATGATGCAGCAGCAAATTACACTGAGGAGCCTCTCCAACTCT3E700[43,45] Xmag905 ATGTGAATGGAAGGTCGGAGAGCACTTGCAGGCTCTTCAT3E260[43-44] SM4E-1ATTCCTAATGGTCCTACAGC ATCACGCACGAACAAGTC 4E580[40] Xmag1682 CGAATGCCAAGCTGTTCCCTACATGCCCCTTGAGAGTGTGG4E855[43-44] Xmag2055 TTCCCAGCAAAGTAGCACAATGTGGGATCGCCTAGAAATC4E550[43-44] Xmag3733 CACACAACTCCACGGAACAGCATAAACCAGCAGCCCAACT4E&6E300[43-44] Z8-4E374TGTTGCCAGTGGAGGAAGTTGAACTACTGAACCGAAT4E368[47] TNAC1503TGAGGTTGGTTCTCATCTGGACGTTGGAAACAATCTGAATGG5E700.00[43,45] TNAC1559AAACAAGGCCCTGAAACACTTCATTGTCAGGCTATGGGACAT5E1180[43,45] Xmag1293 GATTGTTCTTGGGGAGATGCTTGGCCTTTTGGACCGTCAGAGAC5E260, 300[43-44] Xmag1426 GCGAGTTTTCGTAGCAAAGGTCACAGGAGTGGAGGCTCAC5E650, 280[43-44] Xmag1579 AAGGCATTTTTCTCGGTCTCCACACTTCTTGAGGATTTGAGC5E550[43-44] Xmag4013 TCGACTCCCATCCTCAAGACCACCACCATTCCTTTGGAG5E650, 400[43-44] Xmag532 CGACGATCCAACCAGCTAATTAGCCAGCCTATGGTAACGG5E250[43-44] Xmag620 TAGTTGCATGGTCGCTTCTGCGTAGCTTTTCGTTGATCCC5E295[43-44] Xedm54CGTCCTTGTTCTTGCTTCAATGAGCAGGCCATAAGGAAAC5ES150[39] Xedm205AGTCGCCGTTGTATAGTGCCCGACCCGGTTCACTTCAG5ES240[39,49] Xgwm205CGACCCGGTTCACTTCAGAGTCGCCGTTGTATAGTGCC5ES180[39] Xedm46CCGCCGTCCGTCCTGCAGGAGGCCTCGTTGTAG5EL & 7EL215[39] Xedm335CGGTCCAAGTGCTACCTTTCCGTACTCCACTCCACACGG5EL & 7EL150[39,44] Xgwm335CGTACTCCACTCCACACGGCGGTCCAAGTGCTACCTTTC5EL & 7EL150[39] TNAC1674CCACCACAGAAGCAGATGAATGCTAGATGGCACACCAAGTG6E700[43,45] Xedm140CCTCCGACGAGATCTGCTACGCTTTGGCTTCCGGTACG6E271[39] Xgwm179AAGTTGAGTTGATGCGGGAGCCATGACCAGCATCCACTC6E162[39] Xmag2276 CCGCCCCGATACAGTTTATTTCTACCGCTCCGTCGTCAACTACTG6E320[43-44] Xmag3017 GGTGAGGGAGTGCGAGAATAGGCTTCCTGTTGCTGTGG6E260, 170[43-44] Xmag3113 TCATGCACCCATCTGGACTAAGCTATTCGTGGCAAACACA6E210[43-44] Xedm149ATCCACGCCAAGCAGAAGCTGTGGGAAGAAGTGCCTTG6EL186[39] Xedm80ATGCTCAAGCCGAGGAAGTATTGATGGAGCGACTGAAATG6ES126, 150[39] Xgwm149CATTGTTTTCTGCCTCTAGCCCTAGCATCGAACCTGAACAAG6ES85[39] Xgwm292TCACCGTGGTCACCGACCCACCGAGCCGATAATGTAC6ES163[39] Xedm158CTACGTCGTCTACCGCTGCTCTTGGGCATCATCGATCTTC7E120[39] Xbarc76ATTCGTTGCTGCCACTTGCTGGCGCGACACGGAGTAAGGACACC7EL210[49] TNAC1825GAAGCGGTTCAGGGTGACACCCATCACGTTGCTGTAGTC7EL750[43,45] TNAC1826CACATATGATGATGACGGCAATGGCAGGGAGGAAACTCTACTG7EL152.00[43,45] TNAC1829GCCACTTCCTCCCTCCTCGTCGGTCCTCCAGTATCAGC7EL210[43,45] TNAC1834CCGAGTGTCATCGTTAGGAAACCAGCAATCCTACCTGTTGAA7EL1900[43,45] TNAC1845AATGAACAGCTTGCTTTCTGCCAGATGCTCTGGATTTCATGG7EL120, 90[43,45] TNAC1956ACGAAGGACAATTGCTGCTAAGTGCACTTCTTGCCCTACTTG7EL1074[43,45] TNAC1957TCAACATTTGCAGGATTGTCATTTCACAGGAACCTCTGCATC7EL170[43,45] XBE399084CCTACCTACGACGCAAGTCCAGCGAGCAGAAAGCATCAAG7EL132[13] Xcfa2040TCAAATGATTTCAGGTAACCACTATTCCTGATCCCACCAAACAT7EL280, 240[49] Xcfa2106GCTGCTAAGTGCTCATGGTGTGAAACAGGGGAATCAGAGG7EL240[49] Xcfa2240TGCAGCATGCATTTTAGCTTTGCCGCACTTATTTGTTCAC7EL250[49] Xedm105ACCGCCAGGGAGCTCTGCGATGTCCTTCTGGCCGTACT7EL240[39] Xedm154GGAGAGGCCACTGTTTTTGAGGCACATCACTCCCTTCTTC7EL230[39] Xgwm130AGCTCTGCTTCACGAGGAAGCTCCTCTTTATATCGCGTCCC7EL250[49] Xgwm295CGTACTCCACTCCACACGGCGGTCCAAGTGCTACCTTTC7EL288[49] Xgwm333GCCCGGTCATGTAAAACGTTTCAGTTTGCGTTAAGCTTTG7EL300[49] Xgwm344CAAGGAAATAGGCGGTAACTATTTGAGTCTGAAGTTTGCA7EL130[49] Xgwm573AAGAGATAACATGCAAGAAATTCAAATATGTGGGAACTAC7EL182[49] Xgwm635TTCCTCACTGTAAGGGCGTTCAGCCTTAGCCTTGGCG7EL130[49] Xksum052 GTCGCTGAGTGCGAACTCCG CAGAGGAAGCCGTCTACTGG 7EL240[49] Xmag1759GTACGAGTATGGCAACAGCCCAACTCTAGGGGCAGATG7EL620, 250[13,43-44] Xmag1932TGGCGTCCTGCTGAAAACCCGAGCACAAACCCTTGG7EL285,235[13,43-44] Xmag2931GGGGGCAGAACTTGTATGAATCTTGAGGGACGTGAGGAAC7EL300[13,43-44] Xmag3283TGCCTGACCTACTGCAACACGAATAGCCATCAACCGCAAG7EL1820[13,43-44] Xpsp3003GATCGACAAGGCTCTAATGCCAGGAGGAGAGCCTCTTGG7EL200[13] Xpsp3123AGATTAGATGGCATAAGCAAAGAGCGATTAATCACCGCCCTTGGTCC7EL120[49] Xpsr680 GCTCCACTACTTCATCATCC TGTTTCCTCTATCACTGACTTG 7EL246[49] XsdauK107AAGACGCAGTGTCTCGAGGTTGCATCCTCAACATTCTCCA7EL100[13,48] XsdauK116TCTGCGCCTTGTATCCTTCTGGAGTTCCTGGATGTCGTGT7EL230[13,48] XsdauK118GTTCAGTTCCACGCCCAATAATTATGCGCCGGGCTTCT7EL170[13,48] XsdauK124CAGTGCTGCAGGAGCTACAAAGAACCCTTCGGTGGAAACT7EL500[13,48] XsdauK13GCCCTGTTACAGTCCACGATGAGCTCCTTGCTCTGATTGG7EL300[13,48] XsdauK130TCACACAACCTGCATTGGATATGCCATACGCATTTCCAG7EL900, 800[13,48] XsdauK138GCAACCCAGTACGCCTAGTTCAAATTCGAAAGCCCACATT7EL400[13,48] XsdauK140AGGGTCTGTTTGGTTGGATGAGGCGTGAGAAGTCAATGCT7EL390[13,48] XsdauK142GTCTCGTTGCAATTGAGGTGGAGGCGAGCTGGAAATACAA7EL375[13,48] XsdauK144GGGCAGGAAAGACAACACTTTTCCCAGCTTTGCTCTTCAT7EL440[13,48] XsdauK159GTCTCGTTGCAATTGAGGTGGAGGCGAGCTGGAAATACAA7EL370[13,48] XsdauK165ACTGCGTCAGATCCGAAGTTTGCAGCAATGAAGTCAGGAA7EL1600[13,48] XsdauK176AGGAGGCCTTCATGCAGATATCGCCATGACTTCCTCTTCT7EL775[13,48] XsdauK27TAAGGTGGCTCCGTTGTCTCCCTCCAGAGCCTCTTTCTCC7EL465[13,48] XsdauK32AGGCCGGAGTCGATTCTATTATCACAGTGCGCTCATCAAA7EL250[13,48] XsdauK336TCATGTATTTGGACAATGTTAAACGAGACAGCTCCATCGGAAAAA7EL400[13,48] XsdauK339CAATTTGCTTCAAGGGGCTATTGGGCTCTTCTTCCCCTAT7EL425[13,48] XsdauK340ACACTCATGGGAGGACGAAGGCTACTAGCAATGGCGGAAC7EL357[13,48] XsdauK341TCAGTCCCGTCACTGTCAACGTCGCCTGCAGGTAGGAGTA7EL250[13,48] XsdauK342TTTACCAAGTGGGTGGAAGCTCCATAAAACGGAAGGCAAC7EL325[13,48] XsdauK343ACGAGGGTGGGAAGAAAGATAGCCAATTTCGCTGCTTAAA7EL324[13,48] XsdauK345GAAACTAGGGGGTCGGGTAGTCAATTTACCAGGGGCATTC7EL500, 410[13,48] XsdauK348GCCAGATAAGAACCCCGTTTGTGGTAATTTTTAGGGAGTGC7EL280, 145[13,48] XsdauK350GAAAGGCAACGAAAATGGAAGGTACATGATTTCCCCATGC7EL240[13,48] XsdauK351AGTCACCCACAAGGACCAACTGGTGCCCATCTTCTGGTAT7EL775[13,48] XsdauK352CTTTCCAACGCCGTATCATTATTGCGCTGACTGTTGTTTG7EL440[13,48] XsdauK353GGCAGGCACCATATTGAAGTTTGCCAACCACGTAGTGAAA7EL272[13,48] XsdauK355GCGAGAACAATAGCCTGATTTTTTTAATGCATGTTCAGCATTTTT7EL400[13,48] XsdauK356ATAGGTTTCTCCCGGCTTTGAAATCTTCACGAATTCAAAACA7EL140[13,48] XsdauK36CCCTGCATGAGTTGTCAATGCATCCTCCAAGGTCATCACA7EL286[13,48] XsdauK38GAAGATGGCGAAGGTGTAGCTTACACTGTCATCGCCGTCT7EL345[13,48] XsdauK4CATCAAGTACGGCGTCGAGATCGGTCACAAGGCTATTCG7EL745, 640[13,48] XsdauK45GGGTTCCTGCTCAAGTTCCTGGCCTCCTTATGACCACAAA7EL860[13,48] XsdauK6CGCGCTCTTCTACTGGTTCTGTAGAAATCGTGGCCCTTGA7EL555[13,48] XsdauK60TGGCGAAGATTTCTTCAAGTCCTGATCGCACGCTATCATGG7EL430[13,48] XsdauK66ATTTGGCTGCGATCGATAAGCCATCTTGTTGTTGCCCTCT7EL330[13,48] XsdauK71GGAGCCAAATCATCCTTGAATGTGCTTCAGTTTCGACAGG7EL440[13,48] XsdauK75TCTGCACCCTAGTGTTGTGGGATCAGGATCCAATGCAGGT7EL520[13,48] XsdauK8CGAAGCAGAAGCACCTGAAGCGCTGAATCGATTACGGAAC7EL985[13,48] XsdauK99GTGATGGGCAACATGTTCTGCCGGTAATGGAAACCAAGAA7EL700, 660[13,48] Xsews19 AAGGCAATGGAGATGTTCG CCCGTTGATGACCGTGTT 7EL275, 195[49] Xwmc273AGTTATGTATTCTCTCGAGCCTGGGTAACCACTAGAGTATGTCCTT7EL200, 150[49] Xwmc702GAATCACATCGAATGGATCTCAGAGGCCTTTTTCGATATTCTGC7EL194[49] Xwmc606CCGATGAACAGACTCGACAAGGGGCTTCGGCCAGTAGTACAGGA7EL/7ES175[49] Xbarc154GTAATTCCGGTTCCACTTGACATTGGATGGGCAGCTTCAAGGTATGTT7ES520[49] Xbarc70GCGAAAAACGATGCGACTCAAAGGCGCCATATAATTCAGACCCACAAAA7ES100[49] Xcfa2049TAATTTGATTGGGTCGGAGCCGTGTCGATGGTCTCCTTG7ES257[49] Xcfa2174ACGGCATCACAGGTTAAAGGGGTCTTTGCACTGCTAGCCT7ES240[49] Xcfd14CCACCGGCCAGAGTAGTATTTCCTGGTCTAACAACGAGAAGA7ES103[49] Xcfd21CCTCCATGTAGGCGGAAATATGTGTCCCATTCACTAACCG7ES260[49] Xcfd31GCACCAACCTTGATAGGGAAGTGCCTGATGATTTTACCCG7ES340[49] Xcfd66AGGTCTTGGTGGTTTTGGTGTTTTCACATGCCCACAGTTG7ES119[49] Xedm109ATGAGGCACAAGTGGATGGTCTCCTGAAGGGAAAAGCTCA7ES446[39] Xedm16TCACCTAACAGCACCACGAGGCCGAGTACCAGCAGTACCA7ES195[39] Xedm34TTGTTTCTTGGCTGGGTTTCGATCTGCCAAAGCCTTCATAA7ES150[39] Xgwm154TCACAGAGAGAGAGGGAGGGATGTGTACATGTTGCCTGCA7ES325[49] Xgwm156CCAACCGTGCTATTAGTCATTCCAATGCAGGCCCTCCTAAC7ES158[39] Xgwm350ACCTCATCCACATGTTCTACGGCATGGATAGGACGCCC7ES160[49] Xgwm44GTTGAGCTTTTCAGTTCGGCACTGGCATCCACTGAGCTG7ES140[49] Xgwm471CGGCCCTATCATGGCTGGCTTGCAAGTTCCATTTTGC7ES141[15] Xgwm473TCATACGGGTATGGTTGGACCACCCCCTTGTTGGTCAC7ES230, 200[49] Xmag2934TGTTCACAAGCGATTCAAGGAATGCAAGATGGTGGCTTCT7ES280[13,43-44] Xwmc83TGGAGGAAACACAATGGATGCCGAGTATCGCCGACGAAAGGGAA7ES153[49] Xwmc653AGTGTTTTAGGGGTGGAAGGGACGGAACCCTAAACCCTAGTCG7ES190[49] Xwmc809CAGGTCGTAGTTGGTACCCTGAATGAACACGGCTGGATGTGA7ES360[49]
Molecular identification of FHB resistance gene in varieties derivedfrom common wheat-partial amphiploid
LIU XinLun1, WANG Chao1, NIU LiHua1, LIU ZhiLi2, ZHANG LuDe3, CHEN ChunHuan1, ZHANG RongQi1, ZHANG Hong1, WANG ChangYou1, WANG YaJuan1, TIAN ZengRong1, JI WanQuan1
(1College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi;2Seed Administration Station of Chenggu County, Hanzhong 723200, Shaanxi;3Shaanxi provincial Office of Poverty Alleviation and Development, Xi’an 710006)
Fusarium head blight (FHB) is one of the most devastating diseases of wheat.is one of the important source resistance cultivars of wheat disease resistance breeding, with high tolerance to FHB. Three derived varieties of common wheat-Xinong 509, Xinong 511 and Xinong 529 showed moderate resistance to FHB in field. The objective of this study is to clarify the genetic constitution of resistance gene in three varieties.Single drop injection withwas performed to detect the reaction of the three derived varieties of common wheat-to FHB. Specific molecular markers located on homologous group 1 to 7 ofE chromosome were employed to detect the sources of resistance to FHB, screening the three derived varieties of common wheat-, and their major donor parents Xiaoyan 693, Xiaoyan 597 andSpecific molecular markers closely linked with ?ankingwere used to analyze the relationship between the gene conferring resistance to FHB andThree derived varieties were moderately resistant to FHB in the field experiment, and had no much difference compared with the medium-resistant control variety. Seven of the 105 chromosome E specific molecular markers ofcould be amplified polymorphism bands in the three derived varieties and, which located on the chromosome 7E. A total of 97 specific markers located on the chromosome 7E,20 showed specific bands ofin the three derived varieties of common wheat-and their major donor parents Xiaoyan 693, Xiaoyan 597The results indicate that the 89 cM genetic fragment derived from7E chromosome. Of them, six molecular markers (,,,,and), which were located on 7EL 149.00-7EL 153.77, tightly linked to. However,(7EL 153.77),(7EL 154.70),(7EL 156.27),(7EL 158.02),(7EL 158.97) and(7EL 160.00),which are next to, showed no specific bands ofamong the three derived varieties of common wheat-and Xiaoyan 597.Xinong 509, Xinong 511 and Xinong 529, derived fromwith good FHB resistance, possess a novel FHB resistance gene, which derived fromand was different from.
common wheat;;Fusarium head blight;; molecular marker
2017-05-17;接受日期:2017-07-23
國(guó)家重點(diǎn)研發(fā)計(jì)劃(2016YFD0102000)、陜西省科技統(tǒng)籌創(chuàng)新工程計(jì)劃(2015KTZDNY01-01-02)
劉新倫,E-mail:liuxxlun@126.com。王超,E-mail:1240026878@qq.com。劉新倫和王超為同等貢獻(xiàn)作者。通信作者吉萬全,E-mail:jiwanquan2008@126.com
中國(guó)農(nóng)業(yè)科學(xué)2017年20期