家畜iPS技術(shù)研究與應(yīng)用

劉蕾 陳麗文 趙慶國 周光斌 崔春

摘 要：對建立豬和牛的胚胎干細(xì)胞系（Embryonic stem cells，ESCs）的條件進(jìn)行了摸索和優(yōu)化，建立了良好的胚胎培養(yǎng)體系、內(nèi)細(xì)胞團(tuán)分離方法和飼養(yǎng)層的選擇；另外分別建立豬和牛的誘導(dǎo)性多能性干細(xì)胞系（induced pluripotent stem cells，iPS），并分別對其進(jìn)行了鑒定；對目前的培養(yǎng)體系進(jìn)行了優(yōu)化提高了iPS的效率和發(fā)育潛能；嘗試以iPS為供體細(xì)胞進(jìn)行核移植，以期獲得iPS來源的動物種群。

關(guān)鍵詞：豬胚胎干細(xì)胞 誘導(dǎo)多能性干細(xì)胞 重編程

Science，Biejing； 4.China Agricultural University；5.Shanghai Institute of Biological Sciences）

Abstract：Exploration and optimization the conditions for establishment of pigs and cattle embryonic stem cell lines （ESCs） to establish a good embryo culture system， the inner cell mass separation methods and choice of feeder layers. Established pigs and cattle induced pluripotent stem cells （iPSCs）， with respect to their identification； the current training system has been optimized to improve the efficiency and growth potential of iPSCs； try to obtain iPS-derived animal populations， by nuclear transfer that iPS cells as donor cells.

Key Words：Pig Embryonic Stem Cells；Induced Pluripotent Stem Cells；Reprogramming

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