文燕 王星 高盼奇 徐健蓉 陳紅英
摘要:為篩選防曬的天然原料,選擇18種天然藥食植物進(jìn)行防曬能力分析,比較其抗氧化能力、吸收紫外光的能力和抑制酪氨酸酶的活性。結(jié)果表明,玫瑰花、丁香、苦丁茶和綠茶的乙醇提取液具有較強(qiáng)的清除自由基活性,IC50分別為35.00、65.00、60.00、42.00 μg/mL;在乙醇提取液濃度為0.2 mg/mL時(shí),葛根、玫瑰花、黃連、苦丁茶、丁香在紫外中波區(qū)域有較大吸收值,吸收值依次減弱;黃連、苦丁茶、姜黃、玫瑰花、虎杖、槐米、丁香在紫外長(zhǎng)波區(qū)域有較大吸收值,吸收依次減弱。桑白皮、虎杖、紅景天、綠茶、花椒、葡萄子、玫瑰花、槐米、苦丁茶對(duì)酪氨酸酶活性的平均抑制率依次減弱。
關(guān)鍵詞:藥食植物;抗氧化能力;紫外吸收;酪氨酸酶活性
中圖分類號(hào):R282? ? ? ? ?文獻(xiàn)標(biāo)識(shí)碼:A
文章編號(hào):0439-8114(2020)14-0149-04
DOI:10.14088/j.cnki.issn0439-8114.2020.14.030 開放科學(xué)(資源服務(wù))標(biāo)識(shí)碼(OSID):
Abstract: In order to screen the natural raw materials of sunscreen, 18 natural medicine plants were selected for sun protection analysis. The antioxidant capacity, ultraviolet light absorption capacity and tyrosinase inhibition activities of the materials were compared. The results showed that Rosae rugosae flos, Eugenia caryophyllata Thunb., Ilex latifolia Thunb. and green tea had strong scavenging activity of free radical. The IC50 of their extracting solution were 35.00, 65.00, 60.00 and 42.00 μg/mL, respectively. When the concentration of ethanol extracting solution was 0.2 mg/mL, Pueraria lobatae radix., Rosae rugosae flos, Coptis chinensis Franch., Ilex latifolia Thunb. and Eugenia caryophyllata Thunb. had a larger absorption value in the ultraviolet medium wave region, and the absorption value decreased successively. The absorption value of Coptis chinensis Franch., Ilex latifolia Thunb., Curcuma Longa Rhizoma., Rosae rugosae flos, Reynoutria japonica Houtt., Sophora japonica L. and Eugenia caryophyllata Thunb in the ultraviolet long wave region were relatively large, and the absorption was decreased successively. The average inhibition rate of Polygoni cuspidati rhizoma, Reynoutria japonica Houtt., Rhodiolae crenulatae radix, green tea, Zanthoxyli pericarpimum, the seed of Vitis vinifera L., Rosae rugosae flos, Sophora japonica L. and Ilex latifolia Thunb. on tyrosinase activity decreased in turn.
Key words: edible and medicinal plants;antioxidant capacity;ultraviolet absorption;tyrosinase activity
隨著現(xiàn)代生活水平提高,消費(fèi)者越來越關(guān)注天然護(hù)膚產(chǎn)品。從天然植物中提取原料作為護(hù)膚添加劑,要求有高度安全性,而且具有美白、保濕、添香、防曬、殺菌、抗衰老等作用。護(hù)膚品中添加天然植物成分已有文獻(xiàn)報(bào)道,在生活中也有應(yīng)用。為能在同一條件下有效比較不同天然植物的防曬護(hù)膚能力,從中篩選得到安全有效的防曬護(hù)膚原料,從文獻(xiàn)報(bào)道[1-13]具有防曬作用的天然藥食植物中篩選高頻出現(xiàn)或防曬機(jī)制相對(duì)作用較強(qiáng)的18種藥食材料,對(duì)其防曬能力從清除自由基活性、吸收紫外線能力、抑制酪氨酸酶活性等多個(gè)不同機(jī)制進(jìn)行評(píng)價(jià),以期為這方面研究提供參考。
1 材料與方法
1.1 材料與儀器
丁香、苦丁茶、槐米、玫瑰花、姜黃、綠茶、黃連、蘆薈、桑白皮、花椒、虎杖、肉蓯蓉、黃芩、葛根、苦瓜、燕麥草、葡萄子、紅景天18種材料均購于四川綿陽市天城大藥房。DPPH(2,2-二苯基-1-苦基肼)、酪氨酸酶、酪氨酸購于Sigma公司,其他試劑均為分析純。
主要儀器:U-3900紫外分光光度計(jì)(日本日立高新技術(shù)公司);KQ3200E型超聲波清洗器(昆山市超聲儀器有限公司);TP-214電子天平(美國丹佛公司);RE52CS旋轉(zhuǎn)蒸發(fā)器(上海亞榮生化公司);HH-S4數(shù)顯恒溫水浴鍋(金壇市醫(yī)療儀器廠);T6新世紀(jì)紫外可見分光光度計(jì)(北京普析通用儀器公司)。