王俊英, 王華青, 梁曉東, 劉景輝
(1 中國農(nóng)業(yè)科學院生物技術(shù)研究所, 北京 100081; 2 新疆農(nóng)業(yè)科學院糧食作物研究所, 烏魯木齊 830091;3 內(nèi)蒙古農(nóng)業(yè)大學, 呼和浩特 010019)
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水培燕麥根系形態(tài)和氮吸收流量對硝態(tài)氮供應濃度的響應
王俊英1*, 王華青1, 梁曉東2, 劉景輝3*
(1 中國農(nóng)業(yè)科學院生物技術(shù)研究所, 北京 100081; 2 新疆農(nóng)業(yè)科學院糧食作物研究所, 烏魯木齊 830091;3 內(nèi)蒙古農(nóng)業(yè)大學, 呼和浩特 010019)
水培; 燕麥; 根系形態(tài); 氮流量; 硝態(tài)氮
1.1試驗材料
供試材料為來自張家口地區(qū)的3個裸燕麥栽培品種,即壩莜9號、 壩莜3號和200215,由張家口市農(nóng)科院田長葉研究員選育。
1.2材料培養(yǎng)與不同濃度硝酸鹽處理
區(qū)域內(nèi)地表沒有巖漿巖出露。根據(jù)航空物探磁測資料顯示,位于箭豬坡礦床NNE方向3~13km的深部,有隱伏的花崗巖體存在,這個隱伏巖體是五圩地區(qū)巖漿期后熱液多金屬礦床成礦物質(zhì)來源的提供者[6]。
溫室培養(yǎng)為14 h光照(25℃), 10 h黑暗(20℃),濕度80%,冠層光強400 μmol/(m2·s)。
1.3測定項目和方法
數(shù)據(jù)分析使用非損傷微測系統(tǒng)專用數(shù)據(jù)處理軟件Mageflux進行,離子的流速根據(jù)Fick第一擴散定律公式J=-D·(dc/dx) 制作的流速換算表(版本: JCal V3.2.2)計算,流速值J正值為外流,負值為內(nèi)流。
1.4數(shù)據(jù)處理
應用SPSS v16.0 統(tǒng)計分析軟件對所有試驗數(shù)據(jù)進行單因素ANOVA方差分析,差異顯著性水平為P<0.05。
2.1不同硝態(tài)氮濃度對燕麥根系形態(tài)的影響
表1 不同硝態(tài)氮濃度供應下3種燕麥的根系形態(tài)
2.2不同硝態(tài)氮濃度處理下燕麥品種不同直徑范圍內(nèi)根系長度的分布
表2 不同硝態(tài)氮濃度下不同直徑范圍內(nèi)根系長度 (mm)
注(Note): 同列數(shù)據(jù)后不同字母表示處理間差異達5%顯著水平 Values followed by different letters in a column are significant among treatments at the 5% level. 括號內(nèi)數(shù)據(jù)為不同直徑范圍內(nèi)根系長度占總根長的百分比Values in the parentheses show the percentage of the length of root with various diameters in total root length (%).
圖1 不同硝態(tài)氮濃度供應下不同燕麥品種根部的流量變化Fig.1 The flux changes of various oats root under different -N concentration supply
2.4不同燕麥品種的根系氮吸收量
圖2 不同燕麥品種在不同硝態(tài)氮濃度供應下根部流量Fig.2 The flux of various oats root underdifferent -N concentration supply[注(Note): 柱上不同字母表示處理間差異達5% Different letters above the bars mean significant among treatments at the 5% levels.]
3.1不同硝態(tài)氮濃度對燕麥苗期根系生長的影響
[1]楊海鵬, 孫澤民. 中國燕麥[M]. 北京: 中國農(nóng)業(yè)出版社, 1989.
Yang H P, Sun Z M. China oats[M]. Beijing: China Agriculture Press, 1989.
[2]Wang S P, Wang Y F, Schnug E,etal. Effects of nitrogen and sulphur fertilization on oats yield, quality and digestibility and nitrogen and sulphur metabolism of sheep in the Inner Mongolia Steppes of China[J]. Nutrient Cycling in Agroecosystems, 2002, 62: 195-202.
[3]郭孝, 介曉磊, 胡華鋒, 等. 基施硒肥對裸燕麥營養(yǎng)水平的影響[J]. 草業(yè)學報, 2013, 22(1): 53-59.
Guo X, Jie X L, Hu H F,etal. Effects of basal Se fertilizers on nutrition values of naked oats[J]. Acta Prataculturae Sinica, 2013, 22(1): 53-59.
[4]Collins M, Brinkman M A, Salman A A. Forage yield and quality of oat cultivars with increasing rates of nitrogen fertilization[J]. Agronomy Journal, 1990, 82: 724-728.
[5]肖小平, 王麗宏, 葉桃林, 等. 施N量對燕麥“保羅”鮮草產(chǎn)量和品質(zhì)的影響[J]. 作物研究, 2007, (1): 19-21.
Xiao X P, Wang L H, Ye T L,etal. Effect of nitrogen applcation rate on fresh grass yield and quality of Oat “Baoluo”[J]. Crop Research, 2007, (1): 19-21.
[6]周川姣, 肖相芬, 周順利, 等. 施氮量對兩種類型燕麥物質(zhì)積累和產(chǎn)量的影響[J]. 作物雜志, 2009, (3): 58-61.
Zhou C J, Xiao X F, Zhou S L,etal. Effect of nitrogen application amount on matter accumulation and yield of two types oat[J]. Crops, 2009, (3): 58-61.
[7]肖相芬, 周川姣, 周順利, 等. 燕麥氮吸收利用特性與適宜施氮量的定位研究[J]. 中國農(nóng)業(yè)科學, 2011, 44(22): 4618-4626.
Xiao X F, Zhou C J, Zhou S L,etal. Characteristics of N absorption, utilization and optimum N-fertilizer rate based on a long-term fertilization experiment of oats[J]. Scientia Agricultura Sinica, 2011, 44(22): 4618-4626.
[8]Lynch J. Root architecture and plant productivity[J]. Plant Physiology, 1995, 109(1): 7-13.
[9]Sattelmacher B, Gerendas J, Thoms K. Interaction between root growth and mineral nutrition[J]. Environmental and Experimental Botany, 1993, 33(1): 63-73.
[10]Mengel K. Response of various crop species and cultivars to fertilizer[J]. Plant and Soil, 1983, 72: 305-319.
[11]王艷, 米國華, 陳范駿, 張福鎖. 玉米氮素吸收的基因型差異及其與根系形態(tài)的相關(guān)性[J]. 生態(tài)學報, 2003, 23(2): 297-302.
Wang Y, Mi G H, Chen F J, Zhang F S. Genotypic differences in nitrogen uptake by maize inbred lines and its relation to root morphology[J]. Acta Ecological Sinica, 2003, 23(2): 297-302.
[12]樊明壽, 孫亞卿, 邵金旺, 等. 不同形態(tài)氮素對燕麥營養(yǎng)生長和磷素利用的影響[J]. 作物學報, 2005, 31(1): 114-118.
Fan M S, Sun Y Q, Shao J W,etal. Influence of nitrogen forms on oat growth and phosphorus uptake[J]. Acta Agronomica Sinica, 2005, 31(1): 114-118.
[13]Sun J, Dai S, Wang R,etal. Calcium mediates root K+/Na+homeostasis in poplar species differing in salt tolerance[J]. Tree Physiology, 2009, 29(9): 1175-1186.
[14]Luo J, Li H, Liu T,etal. Nitrogen metabolism of two contrasting poplar species during acclimation to limiting nitrogen availability[J]. Journal of Experimental Botany, 2013, 64(14): 4207-4224.
[16]Forde B G, Lorenzo H. The nutritional control of root development[J]. Plant and Soil, 2001, 232(2): 51-68.
[17]Forde B G, Walch-Liu P. Nitrate and glutamate as environmental cues for behavioral responses in plant roots[J]. Plant, Cell and Environment, 2009, 32(6): 682-693.
[18]Walch-Liu P, Forde B G. Nitrate signalling mediated by the NRT1.1 nitrate transporter antagonises l-glutamate-induced changes in root architecture[J]. Plant Journal, 2008, 54(5): 820-828.
[19]陳磊, 王盛鋒, 劉榮樂, 汪洪. 不同磷供應水平下小麥根系形態(tài)及根際過程的變化特征[J]. 植物營養(yǎng)與肥料學報, 2012, 18(2): 324-331.
Chen L, Wang S F, Liu R L, Wang H. Changes of root morphology and rhizosphere processes of wheat under different phosphate supply[J]. Plant Nutrition and Fertilizer Science, 2012, 18(2): 324-331.
[20]Tang Z, Fan X, Li Q,etal. Knockdown of a rice stelar nitrate transporter alters long-distance translocation but not root influx[J]. Plant Physiology, 2012, 160(4): 2052-2063.
[22]Taylor A R, Bloom A J. Ammonium, nitrate, and proton fluxes along the maize root[J]. Plant, Cell and Environment, 1998, 21(2): 1255-1263.
Response of root morphology and N absorption to nitrate nitrogen supply in hydroponic oats
WANG Jun-ying1*, WANG Hua-qing1, LIANG Xiao-dong2, LIU Jing-hui3*
(1BiotechnologyResearchInstituteofChineseAcademyofAgriculturalSciences,Beijing100081,China; 2CropResearchInstituteofXinjiangAcademyofAgriculturalSciences,Wulumuqi830091,China; 3InnerMongoliaAgriculturalUniversity,Hohhot010019,China)
hydroponics; oat; root morphology; N flux; nitrate nitrogen
2015-02-11接受日期: 2015-05-06網(wǎng)絡出版日期: 2015-08-19
現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項資金(CARS-08-B-5)資助。
王俊英(1971—), 女, 內(nèi)蒙古呼和浩特人, 博士, 副研究員, 主要從事作物水肥高效利用機制研究。
E-mail: wangjunying@caas.cn; cauljh@163.com
S512.6.01
A
1008-505X(2016)04-1049-07